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KMID : 0352819890050020013
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Kosin Medical Journal
1989 Volume.5 No. 2 p.13 ~ p.19
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Ureaplasma Infection of Human Chronic Myelogenous Leukemia Cell Line(K-562)
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김광혁/Kim, Kwang Hyuk
유경식/박인달/장명웅/Yoo, Gyung Sig/Park, In Dal/Chang, myung Woong
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Abstract
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K-562 cells were infected with U. urealyticum and incubated in 5% CO2 incubator at 371C for 10 days. At every 1 day intervals, the ureaplasmal numbers in the culture supernatants were calculated by color change units(CCU) and the viable K-562 cells were counted by trypan blue exclusion method.
The results of this study were as follows.
1. Growth of U. urealyticum was enhanced by day 1, but it was decreased from day 2.
2. U. urealyticum inoculated to K-562 cells survived by day 6 or day 9 according to the quantity of inoculum.
3. Growth curves of K-562 cells infected with U. urealyticum were delayed 1 or 2 days at the peak point when compared with non-infected K-562 cells(control). Furtherrnore, ureaplasmal cytopathic effect to K-562 cells was slight.
4. Numbers of viable K-562 cells infected with U. urealyticum were higher than those of non-infection at day 10.
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